Characterization of the human AGE1.HN cell line: a systems biology approach

Background With the emergence of functional genomics approaches in the past decade, powerful tools for the discovery and understanding of cellular mechanisms are accessible. The main purpose of those analyses is to investigate the cell on the level of the transcriptome, proteome and metabolome to retrieve information on possible limitations for growth or productivity. In subsequent steps, these limitations could be addressed by genetic modifications (e.g. overexpression of genes coding for bottle neck enzymes) or supplementation of the media. In this work, we present a systems biology approach for the analysis of the human protein expression cell line AGE1.hn. The focal point of the analyses was on the central energy metabolism, namely glycolysis, TCA and oxidative phosphorylation. In addition to the metabolome analysis transcriptome and proteome data were obtained. Combining the above mentioned techniques we could gather valuable insights in the cellular processes of a human protein production cell line.